Part:BBa_K3670004

xplA: a flavodoxin domain involved in degradation of RDX

This gene was first submitted by the IGEM RUM-UPRM team as a fusion gene with xplB. Due to extensive research, the xplA and xplB genes are now separated. Both are involved in the reductive denitrification and ring cleavage of explosive organic contaminant, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). These genes are derived from a plasmid-borne gene operon of Rhodococcus rhodochrous strain 11Y.

The xplA encodes for the flavodoxin domain fused (at the N-terminus) of a P450 cytochrome. XplA, when produced, promotes the degradation of RDX. Depending on anaerobic or aerobic conditions, RDX converts to MEDINA and 4-nitro-2,4, diazabutana (NDAB) respectively. In either way, nitrite and formaldehyde will form as byproducts.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 516
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]